Willow Coyote-Maestas, PhD, MS

Assistant Professor

Department of Bioengineering and Therapeutic Sciences

[email protected]

1700 4th Street, Rm 401

UCSF Box 2240

San Francisco, CA 94158

United States

Links

UCSF Profiles Coyote-Maestas Lab

Affiliations

Departmental research area

pharmacogenomics and genomics

computational biology and systems pharmacology

Professional background

Degrees

PhD, Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Twin Cities, 2021

MS, Bioinformatics and Computational Biology, University of Minnesota, 2019

BS, Chemistry, Evergreen State College, 2014

BA, Environmental Studies, Evergreen State College, 2014

Research keywords

Molecular Dynamics Simulation
DNA Transposable Elements
Recombination, Genetic
biophysics
Protein Domains
Potassium Channels
Organic Cation Transporter 1
Mutagenesis, Insertional
Protein engineering
proteins
Protein Conformation
Allosteric Site
Bacteriophages
Potassium Channels, Inwardly Rectifying
Mutation, Missense

Honors & Awards

1.	Investigator, Chan Zuckerberg Biohub, 2023-2028
2.	Hanna Gray Fellow, Howard Hughes Medical Institute, 2021-2027
3.	Fellow, Quantitative Biology Institute, 2021-2023
4.	Milne-Brandenbrug Research Award, University of Minnesota, Twin Cities, 2020
5.	Ross A. Gortner Research Award, University of Minnesota, Twin Cities, 2019
6.	Gilliam Fellow, Howard Hughes Medical Institute, 2018-2021
7.	Graduate Research Fellowship, National Science Foundation, 2017-2018
8.	All Nations Louis Stokes Alliances for Minority Participation in STEM, National Science Foundation, 2013-2014

Publications

Naghipourfar M, Chen S, Howard M, Macdonald C, Saberi A, Hagen T, Mofrad M, Coyote-Maestas W, Goodarzi H. A Suite of Foundation Models Captures the Contextual Interplay Between Codons. bioRxiv. 2024 Oct 13.

Estevam GO, Linossi EM, Macdonald CB, Espinoza CA, Michaud JM, Coyote-Maestas W, Collisson EA, Jura N, Fraser JS. Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain. Elife. 2024 Sep 13; 12.

Trinidad DD, Macdonald CB, Rosenberg OS, Fraser JS, Coyote-Maestas W. Deep mutational scanning of EccD3 reveals the molecular basis of its essentiality in the mycobacterium ESX secretion system. bioRxiv. 2024 Aug 24.

Estevam GO, Linossi EM, Rao J, Macdonald CB, Ravikumar A, Chrispens KM, Capra JA, Coyote-Maestas W, Pimentel H, Collisson EA, Jura N, Fraser JS. Mapping kinase domain resistance mechanisms for the MET receptor tyrosine kinase via deep mutational scanning. bioRxiv. 2024 Jul 18.

Rao J, Xin R, Macdonald C, Howard MK, Estevam GO, Yee SW, Wang M, Fraser JS, Coyote-Maestas W, Pimentel H. Rosace: a robust deep mutational scanning analysis framework employing position and mean-variance shrinkage. Genome Biol. 2024 05 24; 25(1):138.

Yee SW, Macdonald CB, Mitrovic D, Zhou X, Koleske ML, Yang J, Buitrago Silva D, Rockefeller Grimes P, Trinidad DD, More SS, Kachuri L, Witte JS, Delemotte L, Giacomini KM, Coyote-Maestas W. The full spectrum of SLC22 OCT1 mutations illuminates the bridge between drug transporter biophysics and pharmacogenomics. Mol Cell. 2024 May 16; 84(10):1932-1947.e10.

Howard MK, Hoppe N, Huang XP, Macdonald CB, Mehrota E, Grimes PR, Zahm A, Trinidad DD, English J, Coyote-Maestas W, Manglik A. Molecular basis of proton-sensing by G protein-coupled receptors. bioRxiv. 2024 Apr 18.

Dawood M, Fayer S, Pendyala S, Post M, Kalra D, Patterson K, Venner E, Muffley LA, Fowler DM, Rubin AF, Posey JE, Plon SE, Lupski JR, Gibbs RA, Starita LM, Robles-Espinoza CD, Coyote-Maestas W, Gallego Romero I. Defining and Reducing Variant Classification Disparities. medRxiv. 2024 Apr 12.

Polacco BJ, Lobingier BT, Blythe EE, Abreu N, Khare P, Howard MK, Gonzalez-Hernandez AJ, Xu J, Li Q, Novy B, Naing ZZC, Shoichet BK, Coyote-Maestas W, Levitz J, Krogan NJ, Von Zastrow M, Hüttenhain R. Profiling the proximal proteome of the activated μ-opioid receptor. Nat Chem Biol. 2024 Sep; 20(9):1133-1143.

Yee SW, Macdonald C, Mitrovic D, Zhou X, Koleske ML, Yang J, Silva DB, Grimes PR, Trinidad D, More SS, Kachuri L, Witte JS, Delemotte L, Giacomini KM, Coyote-Maestas W. The full spectrum of OCT1 (SLC22A1) mutations bridges transporter biophysics to drug pharmacogenomics. bioRxiv. 2023 Jun 07.

Macdonald CB, Nedrud D, Grimes PR, Trinidad D, Fraser JS, Coyote-Maestas W. DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biology. Genome Biol. 2023 02 24; 24(1):36.

Coyote-Maestas W, Nedrud D, He Y, Schmidt D. Determinants of trafficking, conduction, and disease within a K+ channel revealed through multiparametric deep mutational scanning. Elife. 2022 05 31; 11.

Coyote-Maestas W, Nedrud D, Suma A, He Y, Matreyek KA, Fowler DM, Carnevale V, Myers CL, Schmidt D. Probing ion channel functional architecture and domain recombination compatibility by massively parallel domain insertion profiling. Nat Commun. 2021 12 08; 12(1):7114.

Coyote-Maestas W, Fraser JS. ORACLE reveals a bright future to fight bacteria. Elife. 2021 04 15; 10.

Nedrud D, Coyote-Maestas W, Schmidt D. A large-scale survey of pairwise epistasis reveals a mechanism for evolutionary expansion and specialization of PDZ domains. Proteins. 2021 Feb 23.

Coyote-Maestas W, Nedrud D, Okorafor S, He Y, Schmidt D. Targeted insertional mutagenesis libraries for deep domain insertion profiling. Nucleic Acids Res. 2020 01 24; 48(2):e11.

Coyote-Maestas W, Nedrud D, Okorafor S, He Y, Schmidt D. Targeted insertional mutagenesis libraries for deep domain insertion profiling. Nucleic Acids Res. 2020 Jan 24; 48(2):1010.

Coyote-Maestas W, He Y, Myers CL, Schmidt D. Domain insertion permissibility-guided engineering of allostery in ion channels. Nat Commun. 2019 01 17; 10(1):290.

Heal KR, Qin W, Ribalet F, Bertagnolli AD, Coyote-Maestas W, Hmelo LR, Moffett JW, Devol AH, Armbrust EV, Stahl DA, Ingalls AE. Two distinct pools of B12 analogs reveal community interdependencies in the ocean. Proc Natl Acad Sci U S A. 2017 01 10; 114(2):364-369.