Willow Coyote-Maestas, PhD, MS

Assistant Professor

Department of Bioengineering and Therapeutic Sciences

[email protected]

1700 4th Street, Rm 401

UCSF Box 2240

San Francisco, CA 94158

United States

Links

UCSF Profiles Coyote-Maestas Lab

Affiliations

Departmental research area

pharmacogenomics and genomics

computational biology and systems pharmacology

Professional background

Degrees

PhD, Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Twin Cities, 2021

MS, Bioinformatics and Computational Biology, University of Minnesota, 2019

BS, Chemistry, Evergreen State College, 2014

BA, Environmental Studies, Evergreen State College, 2014

Research keywords

DNA Transposable Elements
Recombination, Genetic
biophysics
INDEL Mutation
Protein Domains
Potassium Channels
Mutagenesis, Insertional
Ion Channel Gating
Protein engineering
proteins
Molecular Evolution
Allosteric Site
Bacteriophages
Potassium Channels, Inwardly Rectifying
Mutation, Missense

Honors & Awards

1.	Investigator, Chan Zuckerberg Biohub, 2023-2028
2.	Hanna Gray Fellow, Howard Hughes Medical Institute, 2021-2027
3.	Fellow, Quantitative Biology Institute, 2021-2023
4.	Milne-Brandenbrug Research Award, University of Minnesota, Twin Cities, 2020
5.	Ross A. Gortner Research Award, University of Minnesota, Twin Cities, 2019
6.	Gilliam Fellow, Howard Hughes Medical Institute, 2018-2021
7.	Graduate Research Fellowship, National Science Foundation, 2017-2018
8.	All Nations Louis Stokes Alliances for Minority Participation in STEM, National Science Foundation, 2013-2014

Publications

Polacco BJ, Lobingier BT, Blythe EE, Abreu N, Khare P, Howard MK, Gonzalez-Hernandez AJ, Xu J, Li Q, Novy B, Naing ZZC, Shoichet BK, Coyote-Maestas W, Levitz J, Krogan NJ, Von Zastrow M, Hüttenhain R. Profiling the proximal proteome of the activated μ-opioid receptor. Nat Chem Biol. 2024 Mar 25.

Estevam GO, Linossi EM, Macdonald CB, Espinoza CA, Michaud JM, Coyote-Maestas W, Collisson EA, Jura N, Fraser JS. Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain. bioRxiv. 2023 Aug 03.

Yee SW, Macdonald C, Mitrovic D, Zhou X, Koleske ML, Yang J, Silva DB, Grimes PR, Trinidad D, More SS, Kachuri L, Witte JS, Delemotte L, Giacomini KM, Coyote-Maestas W. The full spectrum of OCT1 (SLC22A1) mutations bridges transporter biophysics to drug pharmacogenomics. bioRxiv. 2023 Jun 07.

Macdonald CB, Nedrud D, Grimes PR, Trinidad D, Fraser JS, Coyote-Maestas W. DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biology. Genome Biol. 2023 02 24; 24(1):36.

Coyote-Maestas W, Nedrud D, He Y, Schmidt D. Determinants of trafficking, conduction, and disease within a K+ channel revealed through multiparametric deep mutational scanning. Elife. 2022 05 31; 11.

Coyote-Maestas W, Nedrud D, Suma A, He Y, Matreyek KA, Fowler DM, Carnevale V, Myers CL, Schmidt D. Probing ion channel functional architecture and domain recombination compatibility by massively parallel domain insertion profiling. Nat Commun. 2021 12 08; 12(1):7114.

Coyote-Maestas W, Fraser JS. ORACLE reveals a bright future to fight bacteria. Elife. 2021 04 15; 10.

Nedrud D, Coyote-Maestas W, Schmidt D. A large-scale survey of pairwise epistasis reveals a mechanism for evolutionary expansion and specialization of PDZ domains. Proteins. 2021 Feb 23.

Coyote-Maestas W, Nedrud D, Okorafor S, He Y, Schmidt D. Targeted insertional mutagenesis libraries for deep domain insertion profiling. Nucleic Acids Res. 2020 01 24; 48(2):e11.

Coyote-Maestas W, Nedrud D, Okorafor S, He Y, Schmidt D. Targeted insertional mutagenesis libraries for deep domain insertion profiling. Nucleic Acids Res. 2020 Jan 24; 48(2):1010.

Coyote-Maestas W, He Y, Myers CL, Schmidt D. Domain insertion permissibility-guided engineering of allostery in ion channels. Nat Commun. 2019 01 17; 10(1):290.

Heal KR, Qin W, Ribalet F, Bertagnolli AD, Coyote-Maestas W, Hmelo LR, Moffett JW, Devol AH, Armbrust EV, Stahl DA, Ingalls AE. Two distinct pools of B12 analogs reveal community interdependencies in the ocean. Proc Natl Acad Sci U S A. 2017 01 10; 114(2):364-369.