Bo Huang, PhD

Professor
Department of Pharmaceutical Chemistry
[email protected]
Phone: +1 415 476-1866
1700 4th Street, Rm 303A
UCSF Box 2532
San Francisco, CA 94158
United States

Links

Huang Lab UCSF Profiles Super-Resolution: Localization Microscopy
Fourier Space: Fourier Transform
Photons: What is Light?
@bohuanglab
Google Scholar

Affiliations

Joint appointments

Department of Biochemistry and Biophysics, School of Medicine

Education programs

UCSF - UC Berkeley Joint Graduate Group in Bioengineering
Biophysics Graduate Program (BP)
Chemistry and Chemical Biology Graduate Program (CCB)
UCSF Tetrad Graduate Program
Herbert W. Boyer Program in Biological Sciences (PIBS)

UCSF centers, institutes, and research programs

QB3
Quantitative Biosciences Institute (QBI)
Chan Zuckerberg Biohub

What I do

I am interested in understanding how macromolecular interactions sets up subcellular compartments that control signaling and nuclear domains that regulate gene function. For this goal, I am developing new fluorescence microscopy techniques and engineering new protein and cellular tools, so that the spatial organization and temporal dynamics of small subcellular structures can be visualized directly.

Departmental research area

physical biology

My research expertise

Protein and cell engineering, fluorescence microscopy

Professional background

Degrees

PhD, Chemistry, Stanford University, 2006
BS, Chemistry, Peking University, 2001

Biography

Cellular processes are carried out by coordinated participation of many biomolecules in a tiny volume. Many people have been dreaming to see clear pictures of these processes in order to understand how these molecules work together. Taking on this challenge, we are developing new visualization techniques and imaging probes by combining super-resolution microscopy, protein engineering and microfluidic automation. We are particularly interested in the following problems:

(1) Physical organization and dynamics of the genome,
(2) Architecture of large protein complexes such as the centrosome, and
(3) Spatial distribution of membrane proteins, particularly G-protein coupled receptors and neuron adhesion molecules, and how this distribution defines their signaling specificity.

In order to study these systems, we are developing the following microscopy technologies:

(1) Super-resolution and light-sheet microscopes that can visualize subcellular structures at a higher spatial resolution, record long term cell behavior, and track cells in intact animals, and
(2) New fluorescent probes based on fluorescent proteins, nanobodies and aptamers so that biological questions can be converted into "imageable" ones.

Research keywords

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • High-Throughput Screening Assays
  • Optics and Photonics
  • Fluorescent Dyes
  • Lighting
  • CRISPR-Associated Proteins
  • Green Fluorescent Proteins
  • Image Processing, Computer-Assisted
  • Luminescent Proteins
  • Fluorescence Microscopy
  • Molecular Imaging
  • Dimethylpolysiloxanes
  • microscopy
  • Silicones
  • HEK293 Cells
1. UCSF School of Pharmacy leads in NIH funding for the 43rd straight year
2. Update from the Dean: Passing the torch
3. UCSF School of Pharmacy leads in NIH funding for the 42nd straight year
4. Faculty members inducted into the Chan Zuckerberg Biohub Investigator Program
5. UCSF School of Pharmacy leads in NIH funding for the 41st straight year
6. Grants & Fellowships: Summer 2018
7. Update from the Dean – March 2018
8. Microscopy illuminates life
9. Huang wins 2016 Early Career Award
10. Huang’s students teach science in an innovative way
11. Huang advances to tenured position as associate professor
12. Update from the Dean - Fall/Winter 2014
13. Gartner receives NIH New Innovator Award to build complex human breast tissue
14. Shu receives NIH New Innovator Award to study protein interactions
15. Update from the Dean - Fall/Winter 2012
16. Huang receives NIH New Innovator Award
17. Searle Scholar awards go to UCSF School of Pharmacy faculty 2 years in a row
18. Update from the Dean - Fall/Winter 2011
19. Huang receives Packard Fellowship
20. Update from the Dean - Fall/Winter 2010
1. UCSF Byers Award for Basic Science, 2017
2. American Society for Cell Biology Young Life Scientist Award, 2016
3. NIH Director's New Innovator Award, 2011
4. Packard Fellowship for Science and Engineering, 2010
5. Searle Scholar, 2010
6. GE & Science Young Life Scientist Award, North America Regional Winner, 2007
7. Annual Reviews Prize in Physical Chemistry, Stanford University, 2006
8. Stanford Graduate Fellowship, 2001-2004

Publications

Foyt D, Brown D, Zhou S, Huang B. HybriSeq: Probe-based Device-free Single-cell RNA Profiling. bioRxiv. 2024 Sep 18.
Meyer K, Yserentant K, Cheloor-Kovilakam R, Ruff KM, Chung CI, Shu X, Huang B, Weiner OD. YAP charge patterning mediates signal integration through transcriptional co-condensates. bioRxiv. 2024 Aug 10.
Haderk F, Chou YT, Cech L, Fernández-Méndez C, Yu J, Olivas V, Meraz IM, Barbosa Rabago D, Kerr DL, Gomez C, Allegakoen DV, Guan J, Shah KN, Herrington KA, Gbenedio OM, Nanjo S, Majidi M, Tamaki W, Pourmoghadam YK, Rotow JK, McCoach CE, Riess JW, Gutkind JS, Tang TT, Post L, Huang B, Santisteban P, Goodarzi H, Bandyopadhyay S, Kuo CJ, Roose JP, Wu W, Blakely CM, Roth JA, Bivona TG. Focal adhesion kinase-YAP signaling axis drives drug-tolerant persister cells and residual disease in lung cancer. Nat Commun. 2024 May 03; 15(1):3741.
Khwaja E, Song YS, Agarunov A, Huang B. CELL-E 2: Translating Proteins to Pictures and Back with a Bidirectional Text-to-Image Transformer. Adv Neural Inf Process Syst. 2023 Dec; 36:4899-4914.
Choi W, Wu H, Yserentant K, Huang B, Cheng Y. Efficient tagging of endogenous proteins in human cell lines for structural studies by single-particle cryo-EM. Proc Natl Acad Sci U S A. 2023 08; 120(31):e2302471120.
Dekker J, Alber F, Aufmkolk S, Beliveau BJ, Bruneau BG, Belmont AS, Bintu L, Boettiger A, Calandrelli R, Disteche CM, Gilbert DM, Gregor T, Hansen AS, Huang B, Huangfu D, Kalhor R, Leslie CS, Li W, Li Y, Ma J, Noble WS, Park PJ, Phillips-Cremins JE, Pollard KS, Rafelski SM, Ren B, Ruan Y, Shav-Tal Y, Shen Y, Shendure J, Shu X, Strambio-De-Castillia C, Vertii A, Zhang H, Zhong S. Spatial and temporal organization of the genome: Current state and future aims of the 4D nucleome project. Mol Cell. 2023 08 03; 83(15):2624-2640.
Khwaja E, Song YS, Huang B. CELL-E: A Text-To-Image Transformer for Protein Localization Prediction. Res Sq. 2023 Jun 02.
Zhao J, Makhija S, Zhou C, Zhang H, Wang Y, Muralidharan M, Huang B, Cheng Y. Structural insights into the human PA28-20S proteasome enabled by efficient tagging and purification of endogenous proteins. Proc Natl Acad Sci U S A. 2022 08 16; 119(33):e2207200119.
Zarei M, Xie D, Jiang F, Bagirov A, Huang B, Raj A, Nagarajan S, Guo S. High activity and high functional connectivity are mutually exclusive in resting state zebrafish and human brains. BMC Biol. 2022 04 11; 20(1):84.
Kim GJ, Mo H, Liu H, Okorie M, Chen S, Zheng J, Li H, Arkin M, Huang B, Guo S. In Vivo Dopamine Neuron Imaging-Based Small Molecule Screen Identifies Novel Neuroprotective Compounds and Targets. Front Pharmacol. 2022; 13:837756.
Cho NH, Cheveralls KC, Brunner AD, Kim K, Michaelis AC, Raghavan P, Kobayashi H, Savy L, Li JY, Canaj H, Kim JYS, Stewart EM, Gnann C, McCarthy F, Cabrera JP, Brunetti RM, Chhun BB, Dingle G, Hein MY, Huang B, Mehta SB, Weissman JS, Gómez-Sjöberg R, Itzhak DN, Royer LA, Mann M, Leonetti MD. OpenCell: Endogenous tagging for the cartography of human cellular organization. Science. 2022 03 11; 375(6585):eabi6983.
Y. Wang, H. Pinkard, E. Khwaja, S. Zhou, L. Waller, B. Huang. Image denoising for fluorescence microscopy by supervised to self-supervised transfer learning. Optics Express. 2021; 29:41303-41312.
Kim GJ, Mo H, Liu H, Wu Z, Chen S, Zheng J, Zhao X, Nucum D, Shortland J, Peng L, Elepano M, Tang B, Olson S, Paras N, Li H, Renslo AR, Arkin MR, Huang B, Lu B, Sirota M, Guo S. A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons. Elife. 2021 09 22; 10.
Kumar D, Rains A, Herranz-Pérez V, Lu Q, Shi X, Swaney DL, Stevenson E, Krogan NJ, Huang B, Westlake C, Garcia-Verdugo JM, Yoder BK, Reiter JF. A ciliopathy complex builds distal appendages to initiate ciliogenesis. J Cell Biol. 2021 09 06; 220(9).
Shi X, Li Q, Dai Z, Tran AA, Feng S, Ramirez AD, Lin Z, Wang X, Chow TT, Chen J, Kumar D, McColloch AR, Reiter JF, Huang EJ, Seiple IB, Huang B. Label-retention expansion microscopy. J Cell Biol. 2021 09 06; 220(9).
Zhao X, Garcia JQ, Tong K, Chen X, Yang B, Li Q, Dai Z, Shi X, Seiple IB, Huang B, Guo S. Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division. Sci Adv. 2021 06; 7(24).
Tulpule A, Guan J, Neel DS, Allegakoen HR, Lin YP, Brown D, Chou YT, Heslin A, Chatterjee N, Perati S, Menon S, Nguyen TA, Debnath J, Ramirez AD, Shi X, Yang B, Feng S, Makhija S, Huang B, Bivona TG. Kinase-mediated RAS signaling via membraneless cytoplasmic protein granules. Cell. 2021 05 13; 184(10):2649-2664.e18.
Makhija S, Brown D, Rudlaff RM, Doh JK, Bourke S, Wang Y, Zhou S, Cheloor-Kovilakam R, Huang B. Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation. ACS Chem Biol. 2021 04 16; 16(4):671-681.
Keenen MM, Brown D, Brennan LD, Renger R, Khoo H, Carlson CR, Huang B, Grill SW, Narlikar GJ, Redding S. HP1 proteins compact DNA into mechanically and positionally stable phase separated domains. Elife. 2021 03 04; 10.
Peng GE, Pessino V, Huang B, von Zastrow M. Spatial decoding of endosomal cAMP signals by a metastable cytoplasmic PKA network. Nat Chem Biol. 2021 05; 17(5):558-566.
Zhou S, Feng S, Brown D, Huang B. Improved yellow-green split fluorescent proteins for protein labeling and signal amplification. PLoS One. 2020; 15(11):e0242592.
Huang X, Shi X, Hansen ME, Setiady I, Nemeth CL, Celli A, Huang B, Mauro T, Koval M, Desai TA. Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. ACS Nano. 2020 10 27; 14(10):13192-13202.
Zhang J, Velmeshev D, Hashimoto K, Huang YH, Hofmann JW, Shi X, Chen J, Leidal AM, Dishart JG, Cahill MK, Kelley KW, Liddelow SA, Seeley WW, Miller BL, Walther TC, Farese RV, Taylor JP, Ullian EM, Huang B, Debnath J, Wittmann T, Kriegstein AR, Huang EJ. Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency. Nature. 2020 12; 588(7838):459-465.
Chaturvedi S, Klein J, Vardi N, Bolovan-Fritts C, Wolf M, Du K, Mlera L, Calvert M, Moorman NJ, Goodrum F, Huang B, Weinberger LS. A molecular mechanism for probabilistic bet hedging and its role in viral latency. Proc Natl Acad Sci U S A. 2020 07 21; 117(29):17240-17248.
Feng S, Varshney A, Coto Villa D, Modavi C, Kohler J, Farah F, Zhou S, Ali N, Müller JD, Van Hoven MK, Huang B. Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Commun Biol. 2019; 2:344.
Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, Lomvardas S, Weiner OD. Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity. Elife. 2019 05 24; 8.
Yang B, Chen X, Wang Y, Feng S, Pessino V, Stuurman N, Cho NH, Cheng KW, Lord SJ, Xu L, Xie D, Mullins RD, Leonetti MD, Huang B. Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution. Nat Methods. 2019 06; 16(6):501-504.
Das S, Extracellular RNA Communication Consortium, Ansel KM, Bitzer M, Breakefield XO, Charest A, Galas DJ, Gerstein MB, Gupta M, Milosavljevic A, McManus MT, Patel T, Raffai RL, Rozowsky J, Roth ME, Saugstad JA, Van Keuren-Jensen K, Weaver AM, Laurent LC. The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research. Cell. 2019 04 04; 177(2):231-242.
Shi X, Garcia G, Wang Y, Reiter JF, Huang B. Deformed alignment of super-resolution images for semi-flexible structures. PLoS One. 2019; 14(3):e0212735.
Wang Y, Yang B, Feng S, Pessino V, Huang B. Multicolor fluorescent imaging by space-constrained computational spectral imaging. Opt Express. 2019 Feb 18; 27(4):5393-5402.
Chen B, Zou W, Xu H, Liang Y, Huang B. Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag. Nat Commun. 2018 11 29; 9(1):5065.
Chow TT, Shi X, Wei JH, Guan J, Stadler G, Huang B, Blackburn EH. Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection. Nat Commun. 2018 09 04; 9(1):3583.
Percharde M, Lin CJ, Yin Y, Guan J, Peixoto GA, Bulut-Karslioglu A, Biechele S, Huang B, Shen X, Ramalho-Santos M. A LINE1-Nucleolin Partnership Regulates Early Development and ESC Identity. Cell. 2018 07 12; 174(2):391-405.e19.
Kelliher MT, Yue Y, Ng A, Kamiyama D, Huang B, Verhey KJ, Wildonger J. Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts. J Cell Biol. 2018 07 02; 217(7):2531-2547.
Sepulveda G, Antkowiak M, Brust-Mascher I, Mahe K, Ou T, Castro NM, Christensen LN, Cheung L, Jiang X, Yoon D, Huang B, Jao LE. Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates. Elife. 2018 04 30; 7.
Schnitzbauer J, Wang Y, Zhao S, Bakalar M, Nuwal T, Chen B, Huang B. Correlation analysis framework for localization-based superresolution microscopy. Proc Natl Acad Sci U S A. 2018 03 27; 115(13):3219-3224.
Viswanath P, Radoul M, Izquierdo-Garcia JL, Ong WQ, Luchman HA, Cairncross JG, Huang B, Pieper RO, Phillips JJ, Ronen SM. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res. 2018 05 01; 78(9):2290-2304.
Citron YR, Fagerstrom CJ, Keszthelyi B, Huang B, Rusan NM, Kelly MJS, Agard DA. The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles. PLoS One. 2018; 13(1):e0190530.
Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B. Improved split fluorescent proteins for endogenous protein labeling. Nat Commun. 2017 08 29; 8(1):370.
Shi X, Garcia G, Van De Weghe JC, McGorty R, Pazour GJ, Doherty D, Huang B, Reiter JF. Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome. Nat Cell Biol. 2017 Oct; 19(10):1178-1188.
Mcgorty R, Xie D, Huang B. High-NA open-top selective-plane illumination microscopy for biological imaging. Opt Express. 2017 Jul 24; 25(15):17798-17810.
Pessino V, Citron YR, Feng S, Huang B. Covalent Protein Labeling by SpyTag-SpyCatcher in Fixed Cells for Super-Resolution Microscopy. Chembiochem. 2017 08 04; 18(15):1492-1495.
Irannejad R, Pessino V, Mika D, Huang B, Wedegaertner PB, Conti M, von Zastrow M. Functional selectivity of GPCR-directed drug action through location bias. Nat Chem Biol. 2017 Jul; 13(7):799-806.
Guan J, Liu H, Shi X, Feng S, Huang B. Tracking Multiple Genomic Elements Using Correlative CRISPR Imaging and Sequential DNA FISH. Biophys J. 2017 Mar 28; 112(6):1077-1084.
van Lengerich B, Agnew C, Puchner EM, Huang B, Jura N. EGF and NRG induce phosphorylation of HER3/ERBB3 by EGFR using distinct oligomeric mechanisms. Proc Natl Acad Sci U S A. 2017 04 04; 114(14):E2836-E2845.
Ong WQ, Citron YR, Sekine S, Huang B. Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe. ACS Chem Biol. 2017 01 20; 12(1):200-205.
Huang B. A microscopic view of the cell. Mol Biol Cell. 2016 11 01; 27(21):3183-3184.
Liu H, Chen S, Huang K, Kim J, Mo H, Iovine R, Gendre J, Pascal P, Li Q, Sun Y, Dong Z, Arkin M, Guo S, Huang B. A High-Content Larval Zebrafish Brain Imaging Method for Small Molecule Drug Discovery. PLoS One. 2016; 11(10):e0164645.
Bai Y, Liu H, Huang B, Wagle M, Guo S. Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish. BMC Neurosci. 2016 09 15; 17(1):63.
Leonetti MD, Sekine S, Kamiyama D, Weissman JS, Huang B. A scalable strategy for high-throughput GFP tagging of endogenous human proteins. Proc Natl Acad Sci U S A. 2016 06 21; 113(25):E3501-8.
Chen B, Guan J, Huang B. Imaging Specific Genomic DNA in Living Cells. Annu Rev Biophys. 2016 07 05; 45:1-23.
Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B. Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18; 7:11046.
Bieling P, Li TD, Weichsel J, McGorty R, Jreij P, Huang B, Fletcher DA, Mullins RD. Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks. Cell. 2016 Jan 14; 164(1-2):115-127.
Chen B, Hu J, Almeida R, Liu H, Balakrishnan S, Covill-Cooke C, Lim WA, Huang B. Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci. Nucleic Acids Res. 2016 05 05; 44(8):e75.
Kamiyama D, McGorty R, Kamiyama R, Kim MD, Chiba A, Huang B. Specification of Dendritogenesis Site in Drosophila aCC Motoneuron by Membrane Enrichment of Pak1 through Dscam1. Dev Cell. 2015 Oct 12; 35(1):93-106.
Ong WQ, Citron YR, Schnitzbauer J, Kamiyama D, Huang B. Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging. Chem Commun (Camb). 2015 Sep 11; 51(70):13451-3.
Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, McManus MT. Biogenesis, delivery, and function of extracellular RNA. J Extracell Vesicles. 2015; 4:27494.
McGorty R, Liu H, Kamiyama D, Dong Z, Guo S, Huang B. Open-top selective plane illumination microscope for conventionally mounted specimens. Opt Express. 2015 Jun 15; 23(12):16142-53.
Puchner EM, Huang B, Gaub HE. Single molecule techniques--applications in biology. FEBS Lett. 2014 Oct 01; 588(19):3519.
Wang Y, Schnitzbauer J, Hu Z, Li X, Cheng Y, Huang ZL, Huang B. Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm. Opt Express. 2014 Jun 30; 22(13):15982-91.
Sun S, Zhou C, Chen S, Liu J, Yu J, Chilek J, Zhao L, Yu M, Vinluan R, Huang B, Zheng J. Surface-chemistry effect on cellular response of luminescent plasmonic silver nanoparticles. Bioconjug Chem. 2014 Mar 19; 25(3):453-9.
McGorty R, Schnitzbauer J, Zhang W, Huang B. Correction of depth-dependent aberrations in 3D single-molecule localization and super-resolution microscopy. Opt Lett. 2014 Jan 15; 39(2):275-8.
Chen B, Huang B. Imaging genomic elements in living cells using CRISPR/Cas9. Methods Enzymol. 2014; 546:337-54.
Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell. 2013 Dec 19; 155(7):1479-91.
Mennella V, Agard DA, Huang B, Pelletier L. Amorphous no more: subdiffraction view of the pericentriolar material architecture. Trends Cell Biol. 2014 Mar; 24(3):188-97.
Puchner EM, Walter JM, Kasper R, Huang B, Lim WA. Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci U S A. 2013 Oct 01; 110(40):16015-20.
Schnitzbauer J, McGorty R, Huang B. 4Pi fluorescence detection and 3D particle localization with a single objective. Opt Express. 2013 Aug 26; 21(17):19701-8.
Huang F, Wang X, Ostertag EM, Nuwal T, Huang B, Jan YN, Basbaum AI, Jan LY. TMEM16C facilitates Na(+)-activated K+ currents in rat sensory neurons and regulates pain processing. Nat Neurosci. 2013 Sep; 16(9):1284-90.
McGorty R, Kamiyama D, Huang B. Active Microscope Stabilization in Three Dimensions Using Image Correlation. Opt Nanoscopy. 2013 Apr 18; 2(1).
Irannejad R, Tomshine JC, Tomshine JR, Chevalier M, Mahoney JP, Steyaert J, Rasmussen SG, Sunahara RK, El-Samad H, Huang B, von Zastrow M. Conformational biosensors reveal GPCR signalling from endosomes. Nature. 2013 Mar 28; 495(7442):534-8.
Stender AS, Marchuk K, Liu C, Sander S, Meyer MW, Smith EA, Neupane B, Wang G, Li J, Cheng JX, Huang B, Fang N. Single cell optical imaging and spectroscopy. Chem Rev. 2013 Apr 10; 113(4):2469-527.
Kamiyama D, Huang B. Development in the STORM. Dev Cell. 2012 Dec 11; 23(6):1103-10.
Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA. Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol. 2012 Nov; 14(11):1159-68.
Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A. 2012 Aug 28; 109(35):13978-83.
Zhu L, Zhang W, Elnatan D, Huang B. Faster STORM using compressed sensing. Nat Methods. 2012 Apr 22; 9(7):721-3.
Beaudoin GM, Schofield CM, Nuwal T, Zang K, Ullian EM, Huang B, Reichardt LF. Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci. 2012 Jan 04; 32(1):99-110.
Kasper R, Huang B. SnapShot: Light microscopy. Cell. 2011 Nov 23; 147(5):1198.e1.
Huang B. An in-depth view. Nat Methods. 2011 Apr; 8(4):304-5.
Huang B, Babcock H, Zhuang X. Breaking the diffraction barrier: super-resolution imaging of cells. Cell. 2010 Dec 23; 143(7):1047-58.
Dani A, Huang B, Bergan J, Dulac C, Zhuang X. Superresolution imaging of chemical synapses in the brain. Neuron. 2010 Dec 09; 68(5):843-56.
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat Cell Biol. 2010 Sep; 12(9):902-8.
Dani A, Huang B. New resolving power for light microscopy: applications to neurobiology. Curr Opin Neurobiol. 2010 Oct; 20(5):648-52.
Huang B. Super-resolution optical microscopy: multiple choices. Curr Opin Chem Biol. 2010 Feb; 14(1):10-4.
Huang B, Bates M, Zhuang X. Super-resolution fluorescence microscopy. Annu Rev Biochem. 2009; 78:993-1016.
Huang B, Jones SA, Brandenburg B, Zhuang X. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods. 2008 Dec; 5(12):1047-52.
Bates M, Huang B, Zhuang X. Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes. Curr Opin Chem Biol. 2008 Oct; 12(5):505-14.
Huang B, Wang W, Bates M, Zhuang X. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008 Feb 08; 319(5864):810-3.
Kim S, Huang B, Zare RN. Microfluidic separation and capture of analytes for single-molecule spectroscopy. Lab Chip. 2007 Dec; 7(12):1663-5.
Huang B, Kim S, Wu H, Zare RN. Use of a mixture of n-dodecyl-beta-D-maltoside and sodium dodecyl sulfate in poly(dimethylsiloxane) microchips to suppress adhesion and promote separation of proteins. Anal Chem. 2007 Dec 01; 79(23):9145-9.
Bates M, Huang B, Dempsey GT, Zhuang X. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science. 2007 Sep 21; 317(5845):1749-53.
Whorton MR, Bokoch MP, Rasmussen SG, Huang B, Zare RN, Kobilka B, Sunahara RK. A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc Natl Acad Sci U S A. 2007 May 01; 104(18):7682-7.
Huang B, Yu F, Zare RN. Surface plasmon resonance imaging using a high numerical aperture microscope objective. Anal Chem. 2007 Apr 01; 79(7):2979-83.
Huang B, Wu H, Bhaya D, Grossman A, Granier S, Kobilka BK, Zare RN. Counting low-copy number proteins in a single cell. Science. 2007 Jan 05; 315(5808):81-4.
Luo Y, Huang B, Wu H, Zare RN. Controlling electroosmotic flow in poly(dimethylsiloxane) separation channels by means of prepolymer additives. Anal Chem. 2006 Jul 01; 78(13):4588-92.
Wu H, Huang B, Zare RN. Generation of complex, static solution gradients in microfluidic channels. J Am Chem Soc. 2006 Apr 05; 128(13):4194-5.
Huang B, Wu H, Kim S, Kobilka BK, Zare RN. Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization. Lab Chip. 2006 Mar; 6(3):369-73.
Wu H, Huang B, Zare RN. Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding. Lab Chip. 2005 Dec; 5(12):1393-8.
Huang B, Wu H, Kim S, Zare RN. Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption. Lab Chip. 2005 Oct; 5(10):1005-7.
Perroud TD, Huang B, Zare RN. Effect of bin time on the photon counting histogram for one-photon excitation. Chemphyschem. 2005 May; 6(5):905-12.
Huang B, Perroud TD, Zare RN. Photon counting histogram: one-photon excitation. Chemphyschem. 2004 Oct 18; 5(10):1523-31.
Perroud TD, Huang B, Wallace MI, Zare RN. Photon counting histogram for one-photon excitation. Chemphyschem. 2003 Oct 17; 4(10):1121-3.
Whelan RJ, Wohland T, Neumann L, Huang B, Kobilka BK, Zare RN. Analysis of biomolecular interactions using a miniaturized surface plasmon resonance sensor. Anal Chem. 2002 Sep 01; 74(17):4570-6.
Ying L, Huang X, Huang B, Xie J, Zhao J, Zhao XS. Fluorescence emission and absorption spectra of single Anabaena sp. strain PCC7120 cells. Photochem Photobiol. 2002 Sep; 76(3):310-3.