Balyn Zaro, PhD

Assistant Professor

Department of Pharmaceutical Chemistry

[email protected]

Phone: +1 415 514-9219

555 Mission Bay Blvd South, Rm 452Q

San Francisco, CA 94158

United States

Links

Zaro Lab

UCSF Profiles

Departmental research area

physical biology

chemical biology and medicinal chemistry

Research keywords

Carbohydrate Metabolism, Deoxyglucose, Dimethyl Fumarate, Acetylglucosamine, Plasma Cells, Proteomics, Post-Translational Protein Processing, Myeloid Differentiation Factor 88, Molecular Probes, Interleukin-1 Receptor-Associated Kinases, Glucosamine, N-Acetylglucosaminyltransferases, Fumarates, Glycosylation, Alkynes

News

Publications

Zaro BW, Noh JJ, Mascetti VL, Demeter J, George B, Zukowska M, Gulati GS, Sinha R, Flynn RA, Banuelos A, Zhang A, Wilkinson AC, Jackson P, Weissman IL. Proteomic analysis of young and old mouse hematopoietic stem cells and their progenitors reveals post-transcriptional regulation in stem cells. Elife. 2020 Nov 25; 9.

Pedowitz NJ, Zaro BW, Pratt MR. Metabolic Labeling for the Visualization and Identification of Potentially O-GlcNAc-Modified Proteins. Curr Protoc Chem Biol. 2020 06; 12(2):e81.

Darabedian N, Yang B, Ding R, Cutolo G, Zaro BW, Woo CM, Pratt MR. O-Acetylated Chemical Reporters of Glycosylation Can Display Metabolism-Dependent Background Labeling of Proteins but Are Generally Reliable Tools for the Identification of Glycoproteins. Front Chem. 2020; 8:318.

Barkal AA, Brewer RE, Markovic M, Kowarsky M, Barkal SA, Zaro BW, Krishnan V, Hatakeyama J, Dorigo O, Barkal LJ, Weissman IL. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy. Nature. 2019 08; 572(7769):392-396.

Senkane K, Vinogradova EV, Suciu RM, Crowley VM, Zaro BW, Bradshaw JM, Brameld KA, Cravatt BF. The Proteome-Wide Potential for Reversible Covalency at Cysteine. Angew Chem Int Ed Engl. 2019 08 12; 58(33):11385-11389.

Zaro BW, Vinogradova EV, Lazar DC, Blewett MM, Suciu RM, Takaya J, Studer S, de la Torre JC, Casanova JL, Cravatt BF, Teijaro JR. Dimethyl Fumarate Disrupts Human Innate Immune Signaling by Targeting the IRAK4-MyD88 Complex. J Immunol. 2019 05 01; 202(9):2737-2746.

Walter LA, Batt AR, Darabedian N, Zaro BW, Pratt MR. Azide- and Alkyne-Bearing Metabolic Chemical Reporters of Glycosylation Show Structure-Dependent Feedback Inhibition of the Hexosamine Biosynthetic Pathway. Chembiochem. 2018 09 17; 19(18):1918-1921.

Bateman LA, Zaro BW, Miller SM, Pratt MR. Correction to "An Alkyne-Aspirin Chemical Reporter for the Detection of Aspirin-Dependent Protein Modification in Living Cells". J Am Chem Soc. 2018 04 11; 140(14):4954.

The New Chemical Reporter 6-Alkynyl-6-deoxy-GlcNAc Reveals O-GlcNAc Modification of the Apoptotic Caspases That Can Block the Cleavage/Activation of Caspase-8. J Am Chem Soc. 2017 06 14; 139(23):7872-7885.

Metabolic Chemical Reporters of Glycans Exhibit Cell-Type-Selective Metabolism and Glycoprotein Labeling. Chembiochem. 2017 07 04; 18(13):1177-1182.

The Small Molecule 2-Azido-2-deoxy-glucose Is a Metabolic Chemical Reporter of O-GlcNAc Modifications in Mammalian Cells, Revealing an Unexpected Promiscuity of O-GlcNAc Transferase. ACS Chem Biol. 2017 03 17; 12(3):787-794.

Metabolically Labile Fumarate Esters Impart Kinetic Selectivity to Irreversible Inhibitors. J Am Chem Soc. 2016 12 14; 138(49):15841-15844.

Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells. Sci Signal. 2016 09 13; 9(445):rs10.

O-GlcNAc modification blocks the aggregation and toxicity of the protein a-synuclein associated with Parkinson's disease. Nat Chem. 2015 Nov; 7(11):913-20.

Chemoproteomics reveals Toll-like receptor fatty acylation. BMC Biol. 2014 Nov 05; 12:91.

Changes in metabolic chemical reporter structure yield a selective probe of O-GlcNAc modification. J Am Chem Soc. 2014 Sep 03; 136(35):12283-95.

Chemical reporter for visualizing metabolic cross-talk between carbohydrate metabolism and protein modification. ACS Chem Biol. 2014 Sep 19; 9(9):1991-6.

Identification of O-GlcNAc modification targets in mouse retinal pericytes: implication of p53 in pathogenesis of diabetic retinopathy. PLoS One. 2014; 9(5):e95561.

An alkyne-aspirin chemical reporter for the detection of aspirin-dependent protein modification in living cells. J Am Chem Soc. 2013 Oct 02; 135(39):14568-73.

Incorporation of unnatural sugars for the identification of glycoproteins. Methods Mol Biol. 2013; 951:57-67.

N-Propargyloxycarbamate monosaccharides as metabolic chemical reporters of carbohydrate salvage pathways and protein glycosylation. Chem Commun (Camb). 2013 May 14; 49(39):4328-30.

Zaro BW, Yang YY, Hang HC, Pratt MR. Chemical reporters for fluorescent detection and identification of O-GlcNAc-modified proteins reveal glycosylation of the ubiquitin ligase NEDD4-1. Proc Natl Acad Sci U S A. 2011 May 17; 108(20):8146-51.

Robust in-gel fluorescence detection of mucin-type O-linked glycosylation. Bioorg Med Chem Lett. 2011 Sep 01; 21(17):5062-6.

Lau HD, Yaegashi J, Zaro BW, Pratt MR. Precise control of protein concentration in living cells. Angew Chem Int Ed Engl. 2010 Nov 02; 49(45):8458-61.