Bo Huang, PhD
What I do
I am particularly interested in understanding the spatial organization of the genome, the architecture of protein complexes, and the regulation of membrane receptor signaling. To answer these challenging questions, we take the approach of developing new techniques that allow them to be studied by direct visualization.
My research expertise
Cellular processes are carried out by coordinated participation of many biomolecules in a tiny volume. Many people have been dreaming to see clear pictures of these processes in order to understand how these molecules work together. Taking on this challenge, we are developing new visualization techniques and imaging probes by combining super-resolution microscopy, protein engineering and microfluidic automation. We are particularly interested in the following problems:
(1) Physical organization and dynamics of the genome,
(2) Architecture of large protein complexes such as the centrosome, and
(3) Spatial distribution of membrane proteins, particularly G-protein coupled receptors and neuron adhesion molecules, and how this distribution defines their signaling specificity.
In order to study these systems, we are developing the following microscopy technologies:
(1) Super-resolution and light-sheet microscopes that can visualize subcellular structures at a higher spatial resolution, record long term cell behavior, and track cells in intact animals, and
(2) New fluorescent probes based on fluorescent proteins, nanobodies and aptamers so that biological questions can be converted into "imageable" ones.